A real time fluorescent PCR(ATF-PCR) method with TaqMan probe that was designed within rDNA-ITS2 was established to detect Pine Wood Nematode, Bursaphelenchus xylophilus.

• 以松材线虫rDNA -ITS2为靶区 ,建立松材线虫的TaqMan探针实时荧光PCR检测方法。