A novel shuttle plasmid which can be replicated in yeast and expressed in mammal cell is required in oral gene therapy with yeast as vector to deliver target gene.

• 为研究酵母作为载体在口服基因治疗及免疫中的作用,需要一种能够在酵母中复制而在哺乳动物细胞中表达的穿梭载体。