A new fluorescence quenching method was developed based on safranine T,a cation fluorescence dye,and used to study hybridization and dissociation of PNA-DNA for the first time.

• 本文建立了一种以阳离子型荧光染料藏红T(ST)作为荧光试剂的荧光猝灭新方法,ST首次被用于PNA-DNA杂交和解链的检测。