A new eukaryotic expression vector-pEF-BOS was used in the transfection of COScell, and the expression was detected by Western-blot and immunoprecipitation.

• 采用一种新的真核细胞表达载体pEF－BOS在COS细胞内表达了gp130和GAM两种分子，并利用免疫沉淀和Western－blot观察了表达结果。