A mouse combinatorial Fab library was constructed by inserting both light chain gene and Fd gene fragments into phagemid vector pComb3, and followed by the help phage VCSM 13 infection. The recombination rates of light chains and Fd fragments were 80%.

• 通过PCR反应和双酶切反应鉴定抗体库的重组率，轻链、重链Fd段基因的重组率均为80%25。 抗体Fab段基因库经过辅助噬菌体VCSM13超感染，成功构建了鼠源性噬菌体Fab抗体库。