A fluorescence in situ hybridization(FISH)procedure was adopted to physical mapping two BAC clones 20M14(27 kb)and 64O9(36 kb)which linked to the brown planthopper resistance gene Bph15,in O. glaberrima Steud. ,O.

• 用覆盖抗褐飞虱基因Bph15的两个BAC克隆,即20M14(27kb)和64O9(36kb)作为探针,对非洲栽培稻、药用野生稻和宽叶野生稻体细胞染色体进行荧光原位杂交(FISH)。