A clear zone surrounding the colony of the recombinant yeast was formed on methanol medium containing tannin indicating the presence of tannase secreted by the recombinant.

• 单宁酶在真核系统的表达表现出很大的潜力，相比天然单宁酶的产量低、生产成本高的缺点，利用高效的表达系统生产重组单宁酶有着广阔的前景。