A botulinum neurotoxin blocked the exocytosis completely but not the vacuole formation. Cytochalasin D, an actin filament modifier, did not affect the formation of vacuoles but prolonged its time course.

• 肉毒杆菌神经毒素可以阻断分泌的过程但是并不能阻断空泡的形成,细胞松弛素D不能阻断空泡的形成,但是可以延长空泡的持续时间。