4.CONCLUSION: A recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani was successfully constructed,and can be expressed stably in the NIH3T3 cells.

• 结论:成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒,并且该基因在NIH3T3细胞中获得了稳定表达。