实验中建立的核酸检测方法能够实现对靶序列的实时检测,检测下限达2.3nmol/L,进行9次杂交检测的变异系数为3.5%,重复进行30次检测的变异系数为14.7%。

• The results showed that detection method with good specificity and sensitivity could realize online detection of target sequence. The system could detect 2.3 nmol/L target sequence, CV value of nine detections was 3.5%25 and that of thirty detections was 14.7%25.