(2) Microcell hybrids resistant to HAT and G418 were obtained and 15 clones were obtained by single-cell isolation cloning. STS-PCR and WCP-FISH proved that human chromosome 8 had been successfully introduced into rat liver cancer cell line C5F.

• （2）人8号染色体对大鼠肝癌高转移细胞系C5F的成功导入改变了C5F的转移表型，提供了人8号染色体上存在肝癌转移抑制基因的直接的功能证据，并为其在人8号染色体上的进一步定位奠定了基础。